 |
|
|
|
Recombinant human Cbp / PAG Source:
Expressed in E. coli. DNA sequence encoding intracellular domain
of human Cbp/ PAG (amino acids 41- 432) was fused with a His tag
at the N-terminus.
Description:
Csk binding protein, also known as PAG (Phosphoprotein
Associated with Glycosphingolipid-enriched microdomains) is a
ubiquitously expressed transmembrane adaptor protein present in
membrane rafts. Cbp/PAG has a pivotal role in the assembly of
negative regulators in these membrane domains.
Cbp/PAG consists of a short extracellular domain (16 to 18 residues), a transmembrane domain (20 residues), and a large cytoplasmic domain (387 to 396 residues); it contains ten tyrosine residues, nine of which are potential substrates for Src kinases, and two proline-rich regions.
Example Data:
Fig. 1. SDS-PAGE
Fig.1. SDS-PAGE Electrophoresis of Cbp / PAG recombinant protein (cat. no. RL-004-C100). 12% separating gel, 6% stacking gel; reducing conditions. Lane 1-5: Cbp / PAG recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng, 100 ng per lane). Staining with Coomasie Blue.
Fig. 2. Western Blotting
Fig.2. Western
Blotting analysis of Cbp /
PAG recombinant protein
(cat. no. RL-004-C100).
Reducing conditions. ________________________________________________________________________________________________________________________
|
|
Send mail to [email protected] with questions or comments about this web site.Copyright � 2009 GENTAUR Last modified: 03/02/10 |
GENTAUR Germany
GENTAUR Italy
Česk� republika
Praha
Danmark
Finland Helsset
Ελλάς Αθήνα
Ireland Dublin
Luxembourg
Magyarorsz�g
Budapest
Nederland
Norge
Oslo
�sterreich
Sverige
Stockholm
Schweiz Z�ri
Canada Montreal
US New York
日本