LAB

Source:

Expressed in E. coli. DNA sequence encoding mouse NTAL (amino acids 30 - 203) was fused with a His tag at the N-terminus.

Description:

Non-T cell Activation Linker (NTAL), alternatively termed as Linker for Activation of B cells (LAB), is a 25 kDa transmembrane adaptor protein present in membrane rafts B cells, mast cells, monocytes and NK cells.

Applications: Loading control Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Applications:

Loading control

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Example Data:

Fig. 1. SDS-PAGE

Fig.1. SDS-PAGE Electrophoresis of mouse NTAL / LAB recombinant protein (Cat. No. RL-005-C100; 12% separating gel, 6% stacking gel; reducing conditions).
Lane 1 - 4: mouse NTAL / LAB recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng per lane). Staining with Coomasie Blue.

Fig. 2. Western Blotting

Fig.2. Western Blotting analysis of mouse NTAL / LAB recombinant protein (Cat. No. RL-005-C100). SDS-PAGE Electrophoresis (12% separating gel, 6% stacking gel; reducing conditions).
Lane 1 - 4: immunostaining with NTAL specific antibody; dilution 2 μg/ml). Detection with Goat anti-mouse/ HRP secondary antibody. Amount of mouse NTAL / LAB recombinant protein loaded on each lane: (1) 50 ng, (2) 20 ng, (3) 5 ng, (4) 1 ng.

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