What is HVJ Envelope (HVJ-E)?
HVJ Envelope is a purified product prepared through complete inactivation of Sendai virus (HVJ*). It is a vesicle in which only the cell membrane-fusing capability of the envelope protein of Sendai virus is retained.

It is known that the HN protein in the tunica externa of the Sendai virus recognizes the receptor (possessing sialic acid at the terminal of sugar chain) on the cell membrane and adsorbs it, leading to the induction of membrane fusion mediated by F protein (another component of the envelope). The genomic RNA of the Sendai virus contained in HVJ-E has been inactivated completely and has neither infective nor proliferative potential in humans or experimental animals. HVJ-E can be used safely at ordinary laboratories, without requiring any special operations or facilities *HVJ：Hemagglutinating Virus of Japan

Cmparison with PEG method in hybridoma preparation

	hybridoma growth supplement	hybridoma positive rate	antibody-production positive rate
GenomONE� - CF EX	－	38/96 (40%)	9/96 (9%)
	＋	96/96 (100%)	96/96 (100%)
PEG1500	－	3/96 (3%)	1/96 (1%)
	＋	36/96 (38%)	9/96 (9%)

GenomONE - CF _EX produces more antibody secreting hybridomas than PEG. (following cell fusion, cells were grown in media containing hybridoma growth supplement as indicated).

Normal BALB/c mouse splenocytes (1x10^8 cells) not sensitized with antigen were fused to X63-Ag8.653 myeloma cells (1x10^7 cells) using GenomONE(tm)-CF _EX  or PEG1500. The fused cells obtained with each agent were inoculated onto five 96-well plates (Day 0). Beginning the following day, half of the culture medium (10%FBS/RPMI1640) was replaced with HAT medium at five points of time (Days 1, 2, 3, 5, and 8), and the growth of colonies in each well was assessed on Days 10 -11 to determine the hybridoma-positive rate
(an indicator of efficiency of fusion). On Day 12, mouse antibody level (IgG + IgA + IgM) in the supernatant was measured by ELISA, to calculate the antibody production-positive rate. The effect of adding a commercially available hybridoma supplement to the medium after fusion was also assessed (supplement was also added to the HAT medium).

1. Hybridoma preparation
   Cell fusion involving PEG and electrofusion Preparation of monoclonal antibodies Fusion of antigen-sensitized B cells and myeloma cells
   Example (Published research article)
   Generation and Characterization of Novel Monoclonal Antibodies Against Murine and Human TARSH Proteins.
   N. Uekawa et al . Hybridoma (Larchmt), 26, 381-385(2007).PMID:18158782
2. Fusion of suspension cells
   Example (Published research article)
   Enhanced Tumor-Specific Long-Term Immunity of Hemaggluttinating Virus of Japan-Mediated Dendritic Cell-Tumor Fused Cell Vaccination by Coadministration with CpG Oligodeoxynucleotides
   K. Hiraoka et al. J. Immunol.,173, 4297-4307(2004).PMID:15383558

3. Fusion of adherent cell and suspension cell

4. Application in premature chromosome condensation (PCC)
   Detection of chromosomal damage and repair by fusion of different types of cells in different cell phases
5. Research on regenerative medicine and cytotherapy
   Fusion of somatic cells and stem cells, etc.
6. Research on embryonic development/differentiation
   Nuclear transfer (nuclear replacement)
7. GenomONE� - NEO _EX HVJ Envelope Vector Kit
   Unique, efficient transfection of siRNA, DNA, oligonucleotides, proteins.
   Low toxicity with primary cell lines.

GenomONE� - CF EX Kit Contents (for 10 hybridoma fusion experiments)
Freeze-dried HVJ-E	0.26 ml equivalent
HVJ-E suspension buffer	0.5 ml
20x Cell Fusion Buffer	10 ml

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GenomONE-CF EX HVJ-E 1 Vials Cell fusion Reagents

ISK-CF-001-EX

1 SET

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