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Cellufine Phosphate
Cellufine Phosphate is an affinity media designed for concentration, purification of proteins and, enzymes such as nucleic acid related proteins. Base of media is spherical and rigid cellulose functionalized with Phosphate esters.
Chromatography:
Column: 1.6x10cm (20ml) packed with Cellufine Phosphate
Flow rate: 3ml/min( 90cm/h )
Sample: 7.5mg of RusA D70N obtained after Heparin-Sepharose
chromatography
Gradient: 200ml from 0.1 to 1.3M NaCl in 50mM tris-HCl pH 8.0
SDS-PAGE:
Gel: Novex 4-12%BT gel used with MES-SDS running buffer (Invitrogen)
Key for gel: 1 Cell free extract
2 Unbound material from Heparin-Sepharose column
3 RusA sample obtained from Heparin-Sepharose column
4-6 Fractions across the peak eluted from Cellufine Phosphate
7 RusA reference sample
8 Mark 12 MW standard (Invitrogen)
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