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PIG3 (p53-Inducible Gene 3) ELISA Assay Kit (Colorimetric)

PIG3 Assay is a non-isotopic, non-radioactive immunoassay for the quantitation of the p53 inducible gene 3 (PIG-3) - a long-lived reporter of transient p53 activation.1 The assay is suitable for human sera, plasma, tissue culture supernatants and cellular extracts or lysates.

 
Features:

- High sensitivity
- 4 deg C storage with long shelf life
- Non-radioactive
- 2.5 hour protocol
- Suitable for sera, plasma, culture supernatants, cellular extracts/lysates
- HTS compatible format		 Performance Summary:

- Sensitivity: 40 pg/ml
- Recovery: serum, >98%; plasma, >98%
Precision Studies
- Intra-assay C.V.'s: <3% mid-level
- Inter-assay C.V.'s: <4% mid-level
Ordering Information:
 
Cat. No.
X1326K1
X1326k2
x1326k3
 		 Size
1 x 96 wells
2 x 96 wells
10 x 96 wells
For larger quantity pricing, please contact Customer Service

 
 

 

 

 

 

 

 

 

Legend:
PIG-3 assay. Graphs show pooled coefficients of variation and between assay coefficients of variation (top), senitivity of assay with ng/ml of PIG-3 versus absorbance at 450-550 nm (middle) and standard curve with ng/ml of PIG-3 versus absorbance at 450-550 nm (bottom).

Protocol Summary: Not to be used in place of detailed protocol:

1. Equilibrate kit to room temperature

  
2. Add samples and standards to wells
Add detector antibody to wells
 		 � Incubate 2 hours at room temperature
3. Wash 3 times  
4. Add conjugate to all wells � Incubate 30 minutes at room temperature
5. Wash 3 times  
6. Add substrate to all wells � Incubate 30 minutes at room temperature
7. Add stop solution to all wells  
8. Read plate(s) at 450 nm/550 nm

 

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