Feature and Advantages:
1: High signal and low background
- This solution greatly enhances antigen-antibody reactions, yielding signals several ten times higher than obtained with conventional methods.
- Two kinds of solution is optimized for primary and secondary antibodies, which can suppress the background and increase the S/N ratio.
2: Wide versatility
- It is applicable to various assay system involving antigen-antibody reactions such as Western blotting, dot blotting and ELISA.
- It is compatible with all of the detection system involving chemical coloration, chemiluminescence and fluorescence. The composition has no affect on the activity of labeled antibodies such as peroxidase and alkaline phosphatase.
3: Ready to use
- It is supplied at a ready-for-use concentration.
- It can be directly used as an antibody diluent instead of the PBS or TBS diluents used with conventional methods.
Example:
Detection of phosphorylated protein by Western blotting
Fig
1 shows the result for detecting phosphorylated Akt.
Detection of phosphorylated Akt increased as the insulin
concentration increased. There was a clear signal
enhancement and background suppression in the assay
using Can Get
Signal compared with the conventional method using
TBS-T. This result suggest the use of
Can Get Signal
yield higher signals and lower backgrounds in Western
blotting with antibodies against phosphprylated protein
compared with conventional assay methods using TBS-T as
a diluent
(* This data was provided by Dr. Toshihiko Yanagita of
Pharmacology, Faculty of Medicine, University of
Miyazaki)
|
|
Sample: Cultured bovine adrenomedullary cells
1. Control (H2O) |
_________________________________________________________________________________________________________________________
| Can Get Signal Immunoreaction Enhancer Solution | TYB-NKB-101 | 2*250 ML | �364 |
| Can Get Signal Immunoreaction Enhancer Solution | TYB-NKB-101T | 2*50 ML | �188 |
| Can Get Signal for primary antibody | TYB-NKB-201 | 250 ML | �250 |
| Can Get Signal for secondary antibody | TYB-NKB-301 | 250 ML | �250 |