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Introducing Ziva�
Ultrasensitive BrdU Cell Proliferation Assay
Research Use Only
Potential Uses
Cancer
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Detection of proliferating tumor cells or metastatic cells in blood-
Detection of tumor-specific T-cells-
Detection of stimulatory or inhibitory/toxic effects of various compounds/drug candidates on tumor or normal, primary or cell line viabilityImmunology
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Detection of immunity using small numbers of cells-
Early detection of cell proliferation-
Detection of antigen specific T-cell proliferation-
Detection and quantification of the immunoreactivity of lymphocytes, stimulatory mitogens or antigens, after exposure to various cytokines or growth factors. Antigen specificity.Autoimmunity/Transplantation
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Detection of autoreactive T-cells-
Comparison of responses of donor vs host samplesRecombinant Expression Technology
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Detection of transfected cellsStem Cells
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Quality assessment of stem cells under various media conditionsThe Ziva Performance Difference
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200 x more sensitive than 3H-thymidine (the "Gold Standard")-
A broader dynamic range than 3H-thymidine-
A lower limit of detection: 1-4 proliferating cells in a background of 100,000 viable non-proliferating cells-
Simple to use, non-isotopic, 96 microtiter plate, ELISA format-
Assay performance time: < 1 hour-
Flexibility in small sample size (1 to 105 cells) and sample volume (50 μL to 100 μL), are ideal for longitudinal in vivo studies in rodents.
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