Home Up Call me back Contents Contact Immunology / Infectious agents / Cellular Biology / Molecular biology / Instruments / Lentiviral Technology / RNAi Libreries / Stem Cell Research / MicroRNA / Proteomics / Chemicals / Campaign / AntibodiesPCR DNA RNA REAL time PCR Immuno shot Taq DNA Polymerase (with dNTPs) Can Get Signal MagExtractor Magical Trapper Ligation high Realtime PCR PerfectHyb LINEAR NF-κB Clover Direct AteloGene GenomOne Blend Taq Thermo T7 RNA Polymerase PerfectHyb

Google

       Pricelist 2010 

Up
PCR
DNA RNA
REAL time PCR
Immuno shot
Taq DNA Polymerase (with dNTPs)
Can Get Signal
MagExtractor
Magical Trapper
Ligation high
Realtime PCR
PerfectHyb
LINEAR NF-κB
Clover Direct
AteloGene
GenomOne
Blend Taq
Thermo T7 RNA Polymerase

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

52074 Aachen, Germany

 Tel +32 16 58 90 45

Fax +32 16 50 90 45

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 51 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

 

PerfectHyb® Hybridization Solution

This is a premixed hybridization solution for Northern and Southern Analyses containing a hybridization enhancer. It provides simpler, more rapid analysis than with conventional methods. Precipitation occurs easily in some commercially available hybridization solutions due to high viscosity or the addition of salmon sperm DNA. However, PefectHyb® does not require additives and shows no precipitation occurrence at normal temperatures due to low viscosity, making handling simple. Moreover, it is suitable for analysis of non-RI as well as RI-labeled probes such as DIG.

Application:

Northern and Southern Blot Analysis
This solution requires no additives so handling is easy.

1. Northern Blot Analysis Using PefectHyb®
Northern blot analysis was performed with 5 µg of total RNA from HeLa cells. Total RNA was extracted using MagExtractor®-RNA- (Code No. NPK-201), and then electrophoresis performed in acrylamide gel containing 50% formamide for transfer to the membrane.
An internal probe was prepared from [32P]dCTP with transferrin receptor gene as the template using a Prime-It®II Random Primer Labeling Kit (Code No. SC300385). It was then purified with MagExtractor®-PCR & Gel Clean up- (Code No. NPK-601).
Detection was performed with PefectHyb® and other commercially available hybridization solutions and by the conventional method to compare results. The results showed that PefectHyb® provided higher sensitive detection than other solutions

2. Southern Blot Using PefectHyb®
Human genomic DNA VNTR was analyzed.
1 µg Genomic DNA extracted from a human cell line and digested with Hae? was electrophoresed and transferred to a membrane. Dig-labeled polymorphic analysis probe YNH24 (D2S44) was used for detection.PefectHyb® was confirmed to have higher sensitivity and specificity than other companies’ solutions.

Feature and Advantages:

  • High Sensitivity
    This solution contains a hybridization enhancer and generates a strong signal, making it effective for the detection of genes with low expression rates.
  • Optimize Performance of a Broad Range of Applications
    This solution can be used in Northern and Southern blot analysis. It is also ideal for non-RI analysis.
  • Rapid
    Using this solution allows completion of normal hybridization in 1-2 hours.
  • Easy to use
    This solution does not develop crystal precipitation and has a low viscosity at normal temperatures, allowing simple handling. Addition of salmon sperm DNA is also not necessary. Moreover, it allows hybridization and washing procedures at the same temperature and is thus ideal for analysis using hybridization ovens.

One-Point Advice:

  • Use for the mRNA Blotting Membrane
    We recommend that RI-labeled cDNA probes or oligo probes be used in analysis of pre-blotting membranes in consideration of background etc.
  • Detection of the Gene Navigator® cDNA Array System cDNA array
    The use of PefectHyb® Hybridization Solution -filter array- is recommended for detection of the above-mentioned product.

_________________________________________________________________________________________________________________________

PerfectHyb Hybridization Solution TYB-HYB-101 250 ML €355

 

Send mail to webmaster@gentaur.com with questions or comments about this web site.Copyright © 2009 GENTAUR Last modified: 03/02/10