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Human Tumor Necrosis Factor (TNF) alpha ELISA Assay Kit (Colorimetric)

Human Tumor Necrosis Factor alpha (TNFa) ELISA is a non-isotopic immunoassay for the in vitro quantitation of human phospho TNFa in serum, plasma, cell culture supernatants.

 

Features:

- Colorimetric assay
- HTS compatible format
- High sensitivity
- 4 deg C storage with long shelf life
- Non-radioactive
- 5 hour protocol

 
Performance Summary:

- Sensitivity: 20 pg/ml
- Precision: <10% well-to-well variation
- Linearity: Within 5% of expected values
Ordering Information:
 
Cat. No.
X1851K
 
Size
1 Plate
 
For larger quantity pricing, please contact Customer Service

Protocol Summary: Not to be used in place of detailed protocol:

Steps
Incubation
1. Equilibrate kit to room temperature -
2. Add samples and standards to wells 2 hours room temp. (or overnight 4�C)
3. Wash 4x -
4. Add detector antibody to all wells 2 hours - room temp.
5. Wash 4x -
6. Add HRP conjugate to all wells 30 minutes - room temp.
7. Wash 4x -
8. Add substrate to all wells 30 minutes - room temp.
9. Add stop solution to all wells -
10. Read plate at 450 nm/550 nm -

 

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