Conventional non-viral transfection tools, including
cationic lipids, are incorporated into cells through
endocytosis which results in degradation of the
transferred DNA by lysosomes.
On the other hand, HVJ Envelope VECTOR resists
degradation by lysosomes, making it easy to transfer the
specified DNA. Therefore, HVJ Envelope VECTOR yields
highly efficient gene expression.
Sialic acid receptors, which are needed to trigger
binding with HN protein, exist in almost all animal
cells. Thus, HVJ Envelope VECTOR is useful for a wide
range of targets.
exciting transfection kit for cells, tissue, and
strong knockdowns with low pM amounts of siRNA.
Chemical modification of siRNA not required; means
high functional expression..
small and large, linear or circular
enzymes and antibodies retain function and
protocol and literature citations for effectiveness
of molecules transfected into live animals following
direct injection of HVJ-E vector.
transfect multiple molecules simultaneously
GenomONE HVJ Envelope VECTOR KIT is a highly flexible
tool for transfecting a wide variety of molecules (plasmid
DNAs, siRNAs, oligonucleotides, proteins, antibodies
into cultured cells (adherent and non-adherent) and
tissue. GenomONE is useful for transfecting sensitive
primary cells and is further distinguished by its
ability to deliver contents into live animals. Many
literature citations are available for each
Unlike cationic lipids,
delivers the molecule of interest into cells via
membrane fusion, not by endocytosis where cargo may be
degraded by lysosomal enzymes. Since GenomeONE-Neo is a
purified HVJ (Sendai virus) product, prepared from virus
particles completely inactivated for infectious ability
and proliferative potential it is completely safe to use
without special precautions.
GenomONE provides ready-to-use kits containing the
HVJ envelope and required auxiliary reagents
(incorporation enhancer, incorporation reagent,
introduction enhancer, and buffer). Suggested protocols
for all major applications are included.
data of GenomONE-Neo with cationic lipid-based
GenomONE ™ -Neo with cationic lipid-based
reagent for siRNA delivery
1. Phospholamban (PLB) gene knockdown in
primary rat myocardial cell cultures
2:PLB siRNA (10μg )
3:PLB siRNA (2μg)
4:Scramble siRNA (10μg)
Cationic lipid reagent
siRNA (10μg )
2:PLB siRNA (2μg)
3:Scramble siRNA (10μg )
The efficiency of siRNA transfer
into cells was high (80-100%)
with both reagents. However,
compared to cationic lipid,
knock-down of the target protein
more efficiently at lower
concentrations of siRNA.
【Data】Drs. M. Arai and A. Watanabe (Gunma
University Graduate School of Medicine)
et al., J. Mol. Cell. Cardiol., 37 (3),
2 Bin1 gene knockdown in C2C12 (mouse
myoblast cell line) after induction of
Use of conventional
A, B, and C) failed
to exert sufficient
to these products.
【Data】 Drs. C. Kojima* and H. Sabe (Osaka
Bioscience Institute, *Current address:
Osaka Prefecture University)
et al., EMBO J., 23, 4413-4422 (2004).
3 Eg5 gene knockdown in various cell
Eg5-siRNA was incorporated in
each reagent to a final
concentration of 50 nM in a 96-well
plate. Cells of various lines
were treated with the reagents.
Forty-eight hours later, the
percentage of viable cells was
measured by WST-1 assay. Eg5
knockdown resulted in
suppression of cell
proliferation and induced
apoptosis. The finding of a
lower percentage of viable cells
indicated stronger Eg5 knockdown
4 EGFP gene knockdown in monkey ES cells
Biochem. Biophys. Res. Commun., 331,
2. Highly efficient in vivo gene transfer to
the mouse uterus
－Comparison data with cationic lipid-based
Mol. Hum. Reprod., 9, 603-609 (2003)
3.Transfection of neuroprogenitor cells with iron
nanoparticles for magnetic resonance imaging tracking
－Comparison data with cationic lipid-based reagent－
Mol. Imaging Biol., 7, 286-295 (2005).