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Cellufine GCL-2000
Gel Filtration, or size exclusion chromatography, has proven to
be a simple and effective purification method for proteins and other
biomolecules. However, the traditionally slow flow rates and limiting resolving
power of most gel filtration media have restricted its usefulness. These
problems, inherent to the packings, existed not only at the laboratory
preparative scale but especially at process scale. The limited throughput of gel
filtration columns has often created a significant process bottleneck.
Cellufine GCL media overcome these limitations. The mechanical strength of the
spherical cellulose matrix allows high flow rates, even in large industrial
columns.
Features:
� Rigid spherical particles
� Outstanding mechanical strength
� Hydrophilic
� High pore volume
� Broad selection of fractionation range
� Supplied preswollen
� High chemical resistance
� Autoclavable at 121 �C for 30 minutes
Benefits:
� High flow rate compatibility even in large diameter process columns
� May be pumped or stirred without fines generation
� Low non-specific adsorption and high recovery
� Good capacity
� Optimize selectivity to required molecular weight range
� No time-consuming swelling procedures required
� Usable with all common salts, detergents, chaotropic agents, organic solvents
� Depyrogenation
� Sterilizable
� Regulatory Support
Range of Fractionalization:
This media offer an extraordinarily broad selection of fractionation ranges:
from the unique Cellufine GCL-2000 for very high molecular weight protein
complexes. Table 1 shows the exclusion limit for the media.
Pressure Flow Curves:
Protein Recovery:
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