Blend Taq� / Blend Taq� -Plus-
Blend Taq� and Blend Taq�-Plus- are PCR enzymes based on the method described by Barnes et al. By blending Taq DNA polymerase and other DNA polymerase with proofreading activity in optimal proportions, PCR can be performed with high amplification efficiency and elongation. Blend Taq� -Plus - contains another anti-Taq monoclonal antibody, anti-Taq high, so polymerase activity is inhibited at room temperature prior to PCR. This allows Hot Start PCR without any need for extra manipulation, and reduces extra bands, resulting in high specificity PCR. Moreover, the use of PCR products amplified with this enzyme allows TA cloning.
Application:
PCR
Blend Taq� and Blend Taq� -Plus- retain high
amplification efficiency and elongation and have
especially improved detectability.
1. Comparison
of detectability between Blend Taq�, Blend Taq� -Plus-
and other commercially available long PCR enzymes.
PCR was performed with 3.6 kb of Human β-globin gene as
a target, using Blend Taq� and Blend Taq� -Plus - and
another company�s long PCR enzyme. 5-40 ng of genomic
DNA, 10 pmoles of primer, and 1.25 units of the enzyme
in a 50�l PCR reaction system were used. Blend Taq� and
Blend Taq� -Plus- exhibited good amplification results
even if using only 5 ng of genome and higher
amplification efficiency was observed compared to the
other company�s long PCR enzyme.
Feature and Advantages:
-
Excellent
DNA Amplification Efficiency
Blend Taq� and Blend Taq� -Plus- allow efficient PCR with a small amount of DNA template. -
High Elongation
Blend Taq� and Blend Taq� -Plus- are modified enzymes based on the method described by Barnes et al, and have improved PCR elongation. -
Simple
Setting of PCR Conditions
These are PCR enzymes based on Taq DNA polymerase, and allow PCR under the same cycle as Taq DNA polymerase. -
Hot Start
PCR Allows Improved PCR Performance
Blend Taq� -Plus- contains anti-Taq monoclonal antibody so Hot Start PCR can be performed without any need for extra manipulation.
__________________________________________________________________________________________________________________________
| Blend Taq | TYB-BTQ-101 | 1*250 UNIT | �247 |
| Blend Taq -Plus- | TYB-BTQ-201 | 1*250 UNIT | �262 |