Antibodies against DNA Damage
Pyrimidine Dimers (CPDs) [Clone : TDM-2]
Anti (6-4) photoproducts (6-4 PPs) [Clone : 64M-2]
Anti Dewar photoproducts (Dewar PPs) [Clone : DEM-1]
Prolonged exposure to solar UV radiation may result in acute and chronic health effects on the skin, eye, and immune system, including skin cancers. These harmful effects are suggested to deeply relate to DNA damage. The major types of DNA damage induced by solar UV radiation are cyclobutane pyrimidine dimers (CPDs), (6–4) photoproducts (6-4PPs), and Dewar photoproducts (DewarPPs), which are formed between adjacent pyrimidine nucleotides on the same strand of DNA. These helix-distorting DNA lesions are repaired exclusively by nucleotide excision repair system in humans. Mori et al. have developed and characterized monoclonal antibodies specific for CPDs or 6-4PPs (1). Matsunaga et al. have established and characterized monoclonal antibodies against DewarPPs (2). These antibodies enable one to quantitate photoproducts in DNA purified from cultured cells or from the skin epidermis using an enzyme-linked immunosorbent assay (ELISA) and to visualize and measure photoproducts in DNA in cultured cells or the skin using indirect immunofluorescence. Thus, this technology would contribute to understanding of molecular mechanisms of cellular responses to UV and DNA damage in many research fields including cancer research, photobiology, dermatology, ophthalmology, immunology, and cosmetology.
(1) Toshio Mori, Misa Nakane, Tsuyoshi Hattori, Tsukasa
Matsunaga, Makoto Ihara, Osamu Nikaido, Simultaneous
establishment of monoclonal antibodies specific for
either cyclobutane pyrimidine dimer or (6-4)
photoproduct from the same mouse immunized with
ultraviolet-irradiated DNA. Photochem. Photobiol., 54:
(2) Tsukasa Matsunaga, Yuri Hatakeyama, Michi Ohta, Toshio Mori and Osamu Nikaido, Establishment and characterization of a monoclonal antibody recognizing the Dewar isomers of (6-4) photoproducts. Photochem. Photobiol., 57: 934-940 (1993).